Coding
Part:BBa_K5236007:Design
Designed by: Hening Guo, Yangzihan Chu Group: iGEM24_BASIS-China (2024-09-30)
IsPETase W159HF229Y
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 864
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 864
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 864
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 864
Illegal AgeI site found at 627 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
For expressing the sequence, signal peptides and lac operator are suggested to consider when designing a pathway.
Source
Mutation site predicted by AI